ypgQ [2019-07-03 11:43:30]

Mn²⁺-dependent pyrophosphohydrolase, hydrolyzes (deoxy)ribonucleoside triphosphate [(d)NTP] to (deoxy)ribonucleoside monophosphate and pyrophosphate

locus: BSU21890

pI: 5.51

mw: 22.98 kDa

protein length: 205 aa

gene length: 618 bp

function: degradation of excessive or abnormal nucleotides

product: Mn²⁺-dependent pyrophosphohydrolase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.4|Nucleotide metabolism/ other]

Gene

Coordinates: 2,303,920 2,304,537

The protein

Protein family

• DMRL synthase family (according to Swiss-Prot)

[SW|Domains]

• [SW|HD domain] (aa 22 ... 138) [Pubmed|27062940]

[SW|Cofactors]

• Mn²⁺ [Pubmed|27062940]

Structure

• [PDB|5IHY] [Pubmed|27062940]
• [PDB|5DQV] [Pubmed|27062940]
• [PDB|5DQW] (complexed with ADP) [Pubmed|27062940]

Expression and Regulation

Operons

• genes: [gene|9929712AC9E5581DD0EF3213CC49A0E6C8D3087D|bsaA]-[gene|A0CC440185318F7F8665AF7A8279C79A09E35518|ypgQ]-[gene|B42367583867799BD88D737C848E16B9143C5957|ypgR]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A888 (ypgQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/888 NBRP B. subtilis, Japan]
• BKE21890 ([gene|A0CC440185318F7F8665AF7A8279C79A09E35518|ypgQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCCTTCACAAAAACACCCT, downstream forward: _UP4_TAATAGAAGGCTTGGAGCGA
• BKK21890 ([gene|A0CC440185318F7F8665AF7A8279C79A09E35518|ypgQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCCTTCACAAAAACACCCT, downstream forward: _UP4_TAATAGAAGGCTTGGAGCGA

References

• 25005104,27062940